Gone in Seconds: Praxis, Performance, and Peculiarities of Ultrafast Chiral Liquid Chromatography with Superficially Porous Particles
نویسندگان
چکیده
منابع مشابه
Fast and Efficient HPLC Separations With Superficially Porous Particles
The current trend in liquid chromatography is towards the achievement of higher kinetic efficiency and shorter analysis time. Different types of column packings are now available for attaining very fast and high resolution separations without changing instruments, worrying about high backpressure or compromising column longevity. In recent developments of particle technology, the use of superfi...
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The large size and complexity of many proteins constrains the reversed-phase high-performance liquid chromatography packings that are useful for their separation. Wide-pore, superficially porous, silica-based packings with solid 4.5-microm cores and a 0.25-microm porous outer layer (Poroshell) demonstrate a variety of characteristics that are beneficial for the separation of proteins. A shorter...
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Peak parking experiments were conducted to study the chromatographic behavior in a RPLC system consisting of a column packed with superficially porous C(18)-particles and a mixture of methanol and water (70/30, v/v). The values of the surface diffusion coefficient and the retention equilibrium constant of a column packed with superficially porous C(18)-particles were comparable to those of col...
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The compatibility of superficially porous (SP) resin for label-free intact protein analysis with online capillary LC/MS is demonstrated to give improved chromatographic resolution, sensitivity, and reproducibility. The robustness of the platform was measured against several samples of varying complexity and sample loading amount. The results indicate that capillary SP columns provide high loadi...
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ژورنال
عنوان ژورنال: Analytical Chemistry
سال: 2015
ISSN: 0003-2700,1520-6882
DOI: 10.1021/acs.analchem.5b00715